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tenascin c  (R&D Systems)


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    Structured Review

    R&D Systems tenascin c
    Tenascin C, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tenascin+c/Human%2FMouse+Tenascin+C+Antibody/pm41990751-474-30-31
    Average 93 stars, based on 51 article reviews
    tenascin c - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Coevolution of neoplastic and non-neoplastic reactive astrocyte states converges on mesenchymal-like and injury-response programs during murine glioblastoma progression and post-radiotherapy recurrence
    Article Snippet: .. Day 2, cells were treated with one of the following recombinant proteins; 100ng/ml Gas6 (R&D Systems, 986-GS-025/CF), 5μg/ml Tenascin C (R&D Systems, 3358-TC-050) or 25ng/mL HB-EGF (R&D Systems, 259-HE-050/CF) or 100nM, 500nM or 1μM Bemcentinib (R428), a selective Axl-inhibitor. .. For investigation with Gas6 and R428, 96-well plates were coated with poly-orthinine and laminin before cell seeding and cells were additionally treated with or without 4 Gy radiotherapy using a Cix1 x-ray irradiator (Xstrahl) 1 hours after addition of recombinant protein and Axl-inhibitor.

    Article Title: Coevolution of neoplastic and non-neoplastic reactive astrocyte states converges on mesenchymal-like and injury-response programs during murine glioblastoma progression and post-radiotherapy recurrence
    Article Snippet: .. On day 2, cells were treated with one of the following recombinant proteins; 100ng/ml Gas6 (R&D Systems, 986-GS-025/CF), 1μg/ml Tenascin C (R&D Systems, 3358-TC-050) or 25ng/mL HB-EGF (R&D Systems, 259-HE-050/CF) or 100nM Bemcentinib (R428) for blocking Gas6-Axl-signaling. ..

    other:

    Article Title: Oncogenic and tumor-suppressive forces converge on a progenitor niche at the benign-to-malignant transition.
    Article Snippet: Indexed DNA libraries were double-size purified (0.6–0.8X) with SPRI beads and sequenced on an Illumina sequencer (R1 – 26 cycles, i7 – 8 cycles, R2 – 70 cycles or higher) to a depth of >50 million reads per sample (>13,000 reads per cell) at MSK’s Integrated Genomics Operation Core Facility.

    Incubation:

    Article Title: Mechanistic interrogation of mutation-independent disease modulators of RDEB identifies the small leucine-rich proteoglycan PRELP as a TGF-β antagonist and inhibitor of fibrosis.
    Article Snippet: .. Membranes were blocked with 5% non-fat milk powder in TBS for 1 h at room temperature (RT) and incubated overnight with antibodies against: collagen VII N-terminus (pAb-LH7.2, gift from A. Nystr€om, University of Freiburg, Germany [51]), PRELP (H00005549-B01P, Novus Biologicals), tenascin-C (MAB2138, R&D Systems), collagen I (ab138492, Abcam), p-Smad2/ 3 (#8828), Smad2/3 (#8685), bIG-H3 (#5601) from Cell Signaling Technology, periostin (sc-398631), and GAPDH (sc-47724) from Santa Cruz Biotechnology. ..

    Blocking Assay:

    Article Title: Coevolution of neoplastic and non-neoplastic reactive astrocyte states converges on mesenchymal-like and injury-response programs during murine glioblastoma progression and post-radiotherapy recurrence
    Article Snippet: .. On day 2, cells were treated with one of the following recombinant proteins; 100ng/ml Gas6 (R&D Systems, 986-GS-025/CF), 1μg/ml Tenascin C (R&D Systems, 3358-TC-050) or 25ng/mL HB-EGF (R&D Systems, 259-HE-050/CF) or 100nM Bemcentinib (R428) for blocking Gas6-Axl-signaling. ..

    Staining:

    Article Title: Oncogenic and tumor-suppressive forces converge on a progenitor niche at the benign-to-malignant transition.
    Article Snippet: After washing, nuclei were resuspended in PostHybridization Resuspension Buffer, filtered through a 30 μm Miltenyi Biotec filter, and counted to determine the appropriate volume for loading onto the Chromium X instrument. .. Unconjugated primary antibodies were used in the first staining round, followed by secondary antibody staining: GFP (Abcam, ab13970; RRID:AB_300798, 1:1000), HMGA2 (Cell Signaling Technology, 8179S; clone D1A7, RRID:AB_11178942, 1:500) and Tenascin-C (R&D systems, MAB2138; RRID:AB_2203818, 1:250). .. After imaging, dye inactivation was performed using 0.1 M Na2CO3 3% H2O2 solution for 15 min at room temperature, followed by 1 h blocking with rabbit serum (Sigma-Aldrich, R9133) at room temperature and washing with 1X PBS-T, before starting the next round of AF imaging and staining.



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